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RASTRUM Cell Model Architecture

Ready-to-use cell model architectures optimized for your applications

Empower your research with a range of 3D cell model architectures tailored to your needs. Seamlessly integrate these flexible architectures into various well-plate formats and applications, including imaging, screening, and cell extraction. 

Lung_NHLF_IM_Inset

Imaging Model

A small single-matrix architecture optimized for fast imaging and minimal cell usage. This model ensures clear imaging and compatibility with standard techniques, enabling seamless integration into your workflows. Compatible with 96-well plates on RASTRUM™ and RASTRUM Allegro.

Applications

  • Brightfield and immunofluorescence imaging
  • Drug screening
  • Biochemical assays

 

Features

  • Thin architecture for rapid, high-content imaging
  • Compact design minimizes the number of cells required while maintaining reproducibility

 

Figure Legend: A lung cancer co-culture model made with RASTRUM Allegro. Primary normal human lung fibroblasts (NHLFs) were printed with primary Human Umbilical Vein Endothelial Cells (HUVECs) and A549 lung adenocarcinoma cells using RASTRUM’s Imaging Model architecture. Cells were cultured for 7 days and stained using a PhenoVue Cell Painting Kit (Revvity). Cell nuclei (cyan) were labeled with Hoescht, filamentous actin (yellow) with phalloidin, and membrane glycoproteins (magenta) with concanavalin A. Inset: Imaging Model architecture illustration.

Large_Plug_Model_Data_Architecture

Large Plug Model

A single-matrix architecture tailored for expansion and bulk downstream analyses, delivering the cellular material you need for comprehensive studies and high-throughput applications. Compatible with 96-well plates on RASTRUM and RASTRUM Allegro.

Applications

  • DNA, RNA, and protein analysis
  • Flow cytometry
  • Omics analyses

 

Features

  • Larger matrix architecture for increased cellular material
  • Optimized for workflows requiring bulk analysis and high data yield

 

Figure legend. Liver co-culture created with RASTRUM. Immortalized hepatocyte and stellate cells were printed in a Large Plug Model and cultured for 5 days before treatment with TGF-β1. On Day 7 cells were retrieved from the matrix and RNA isolated. Gene expression of profibriotic markers for Collagen-type 1 (COL1A1) and connective tissue growth factor (CTGF) were shown to be significantly increased following TGF-ꞵ1 treatment.

High-throughput@2x-100

High-Throughput Model

A single matrix model designed for high-throughput in 384-well plates.

  • Drug screening
  • High-content imaging
  • Biochemical assays
  • Consistent and reproducible 3D models in 384-well plate format
Triple matrix@2x-100

Triple Matrix Model

A triple matrix model designed for recapitulating tissue architecture.

  • Migration and invasion
  • Tissue architecture recapitulation
  • Cell signaling and interaction
  • Easy to image cell signaling and interaction
  • 3D models with defined tissue architecture
Double matrix@2x-100

Double Matrix Model

A double matrix model designed for recapitulating tissue architecture.

  • Migration and invasion
  • Tissue architecture recapitulation
  • Cell signalling and interaction
  • Easy to image cell signalling and interaction
  • 3D models with defined tissue architecture
Removable@2x-100

Removable Model

A large removable model for 24-well plates printed on coverslips designed for implantation.

  • Implantation
  • Immunohistochemistry
  • Spatial biology
  • Easy to remove for microscopic imaging
  • Easy to handle for embedding and sectioning