PDAC-IO Validated Solution Data Pack

Overview

 The PDAC-IO Validated Solution is part of the RASTRUM Validated Solution offering. It adds pre-activated human CD8+ T cells to the PDAC Validated Solution, a 3D PANC-1/CAF tumor-stroma model built on the RASTRUM Allegro platform, so teams can study tumor-stroma-immune interactions and responses to checkpoint inhibitors. This Data Pack contains example data for the model: model specifications, imaging, RNA-seq pathway analysis and responses to reference treatments. 

What’s included

• Model specifications: matrix, cell densities and ratio, T cell number, culture media and reference compound concentrations
• Experimental timeline from printing to analysis
• Representative immunofluorescence images of cancer cells, CAFs and CD8+ T cells in the tri-culture
• Bulk RNA-seq pathway analysis comparing tri-cultures with co-cultures of PANC-1 and CD8+ T cells
• Cancer cell death after anti-PD-1, anti-PD-1 plus anti-LAG-3, or anti-PD-1 plus galunisertib, compared with an IgG4 isotype control