PDAC-IO Validated Solution Protocol Pack

Overview

 The PDAC-IO Validated Solution is part of the RASTRUM Validated Solution offering. It adds pre-activated human CD8+ T cells to the PDAC Validated Solution, a 3D PANC-1/CAF tumor-stroma model built on the RASTRUM Allegro platform, so teams can study tumor-stroma-immune interactions and responses to checkpoint inhibitors. This Protocol Pack contains protocols for model generation, CD8+ T cell activation, and T cell and treatment addition, plus supplementary protocols for immunofluorescence staining, T cell labeling and RNA preparation. 

Protocols included

• Standardized generation of the PDAC-IO tumor-stroma model: PANC-1 cancer cells with primary CAFs
• CD8+ T cell revival and activation
• T cell and treatment addition
• RNA preparation for sequencing (supplementary, optional)
• Immunofluorescence staining for cleaved caspase-3 and pan-cytokeratin (supplementary)
• CellTrace Yellow labeling of T cells (supplementary, optional)
  
  

The Protocol Pack also lists materials, cell conditions, plate plans and expected results.