Building the Tumor Microenvironment in 3D: Multicellular Models for Anti-Cancer and Immunotherapies
Watch the on-demand presentation from Matthieu Spriet PhD exploring how 3D multicellular models can be used to study immune cell infiltration, tumor-stroma interactions, and therapeutic response in cancer research.
The tumor microenvironment is shaped by interactions between cancer cells, immune cells, stromal cells, and the surrounding extracellular matrix. These interactions can be difficult to capture in conventional in vitro models, particularly when spatial organization, immune cell migration, and multicellular crosstalk are central to the biology being studied.
In this presentation from the Drug Discovery News Advances in Immuno-Oncology webinar, Matthieu introduces the RASTRUM Platform and shows how researchers are using reproducible 3D cell models to investigate complex tumor microenvironment biology. Examples include models of cytotoxic T cell infiltration into 3D tumor environments, pancreatic cancer co-cultures used to study cancer-T cell interactions, and multicellular models incorporating cancer cells, cancer-associated fibroblasts, and T cells.
The session also highlights work from researchers at the Garvan Institute of Medical Research using RASTRUM Allegro to study how CAFs can influence T cell-mediated cancer cell killing, as well as single-cell RNA sequencing to characterize cancer, fibroblast, and T cell states within multicellular pancreatic cancer models.
What you'll learn:
- How 3D tumor models can be used to study immune cell infiltration and cancer-immune cell interactions
- How matrix and model architecture can support spatial analysis of the tumor microenvironment
- How researchers have used RASTRUM models to investigate antigen-specific T cell interactions with pancreatic cancer cells
- How cancer-associated fibroblasts can influence T cell-mediated cancer cell killing in multicellular pancreatic cancer models
- How single-cell RNA sequencing can be used to characterize distinct cancer, fibroblast, and T cell states within 3D co-cultures
- How the RASTRUM workflow supports reproducible generation of multicellular 3D models for immuno-oncology research
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